Engineering intracellular sialylation pathways

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 9/12 (2006.01) C12N 9/24 (2006.01) C12N 9/68 (2006.01) C12N 9/88 (2006.01) C12N 9/90 (2006.01) C12N 15/09 (2006.01) C12N 15/52 (2006.01) C12N 15/63 (2006.01) C12N 15/866 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2363297

Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.

L'invention concerne des méthodes de manipulation des voies de traitement des glucides dans des cellules étudiées. Ces méthodes consistent à manipuler plusieurs voies impliquées dans la réaction de sialylation par utilisation des techniques de l'ADN de recombinaison et de l'alimentation en substrat, de manière à permettre la production des glycoprotéines sialylées dans les cellules étudiées. Parmi ces travaux de manipulation réalisés sur les glucides, figurent la mise en oeuvre de nouveaux dosages biologiques, l'examen d'une sélection de lignées cellulaires d'insectes et l'utilisation de la bioinformatique pour l'identification de séquences géniques destinées au traitement critique des enzymes. L'invention concerne également des compositions renfermant les cellules étudiées produisant des glycoprotéines sialylées. Ces méthodes et ces compositions sont utilisées pour l'expression hétérologue des glycoprotéines.

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