C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/10 (2006.01) A01H 5/00 (2006.01) C07K 14/435 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12N 9/88 (2006.01) C12N 15/52 (2006.01) C12N 15/62 (2006.01) C12P 21/02 (2006.01) C12Q 1/02 (2006.01) G01N 33/52 (2006.01) G01N 33/566 (2006.01)
Patent
CA 2454899
Recombinant cells which express a fluorescent holo-phycobiliprotein fusion protein and methods of use are described. The cells comprises a bilin, a recombinant bilin reductase, an apo-phycobiliprotein fusion protein precursor of the fusion protein comprising a corresponding apo-phycobiliprotein domain, and a recombinant phycobiliprotein domain-bilin lyase, which components react to form the holo-phycobiliprotein fusion protein. Also described are holo- phycobiliprotein based transcription reporter cells and assays, which cells conditionally express a heterologous-to-the-cell, fluorescent, first holo- phycobiliprotein domain.
Cette invention porte sur des cellules recombinées qui expriment une protéine de fusion holo-phycobiliprotéine fluorescente ainsi que sur des procédés d'utilisation. Ces cellules comprennent une biline, une biline réductase recombinée, un précurseur de protéine de fusion apo-phycobiliprotéine de la protéine de fusion portant un domaine apo-phycobiliprotéine correspondant, ainsi qu'une lyase biline-domaine phycobiliprotéine de recombinaison, lesquels composants réagissent afin de former la protéine de fusion holo-phycobiliprotéine. Cette invention concerne également des cellules et analyses de rapporteurs de transcription à base de holo-phycobiliprotéine, lesquelles cellules expriment de manière conditionnelle un premier domaine holo-phycobiliprotéine fluorescent hétérologue à la cellule.
Cai Yuping
Glazer Alexander N.
Tooley Aaron J.
Battison Williams Dupuis
The Regents Of The University Of California
LandOfFree
Engineering of living cells for the expression of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Engineering of living cells for the expression of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Engineering of living cells for the expression of... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1770504