Engineering protein posttranslational modification in...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/67 (2006.01) A01H 5/00 (2006.01) A01K 67/027 (2006.01) C07K 14/47 (2006.01) C12N 15/09 (2006.01) C12N 15/63 (2006.01) C12N 15/82 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2220109

The invention relates to transgenic non-human multicellular organisms that contain polynucleotides for expressing proteins that alter posttranslational modification. In particular, the invention provides multiply-transgenic animals in which a first transgene encodes a first protein, a second transgene encodes a second protein, and expression of the second protein affects the posttranslational modification of the first protein in cells of said organism. Expression in preferred embodiments is in specific cells and the modified protein is secreted into a bodily fluid. The invention provides related methods, proteins and products. An example provides transgenic animals that express human Protein C and the processing protease PACE/furin in mammary glands and secrete both proteins into milk.

L'invention porte sur des organismes multicellulaires transgéniques non humains contenant des polynucléotides permettant l'expression de protéines qui apportent des changements à une modification post-translative. Cette invention, notamment, concerne des animaux polytransgéniques chez qui un premier transgène code une première protéine, un second transgène code une seconde protéine et chez qui l'expression de la seconde protéine a des incidences sur la modification post-translative de la première protéine dans des cellules dudit organisme. Dans des modes de réalisations préférés de l'invention, l'expression se fait dans des cellules spécifiques et la protéine modifiée est sécrétée dans un liquide organique. L'invention concerne également des procédés, des protéines et des produits afférents. Des animaux transgéniques exprimant, d'une part, une protéine C humaine et la protéase de traitement PACE/furin dans des glandes mammaires et sécrétant, d'autre part, les deux protéines dans du lait constituent un exemple de cette invention.

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