Enhanced 2-keto-l-gulonic acid production

Details Enhanced 2-keto-l-gulonic acid production Enhanced 2-keto-l-gulonic acid production

C - Chemistry, Metallurgy

12

P

C12P 7/60 (2006.01) C07C 59/105 (2006.01) C07K 14/24 (2006.01) C07K 14/245 (2006.01) C07K 14/26 (2006.01) C12N 15/31 (2006.01) C12N 15/52 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2417871

A method for enhancing a host cell's biosynthetic production of 2-KLG is described. Such method comprises selecting a host cell that has an at least partially intracellular synthetic pathway which utilizes 2,5-DKG to produce 2- KLG; increasing the transport of said 2,5-DKG into said host cell while maintaining the integrity of the host cell; culturing the host cell to produce said 2,5-DKG; and producing 2-KLG. The transport of the 2,5-DKG is increased by transforming into the host cell DNA encoding for one or more enzymes transporting the 2,5-DKG into the host cell.

L'invention concerne un procédé destiné à améliorer la production biosynthétique par une cellule hôte d'acide 2-céto-L-gulonique (2-KLG). Ce procédé consiste à sélectionner une cellule hôte comprenant une voie de synthèse au moins partiellement intracellulaire utilisant du 2,5-dicétoglutarate (2,5-DKG) pour produire du 2-KLG, à augmenter le transport de ce 2,5-DKG dans la cellule hôte tout en préservant l'intégrité de cette dernière, à cultiver ladite cellule hôte en vue de produire le 2,5-DKG, puis à produire du 2-KLG. On améliore le transport du 2,5-DKG en transformant, dans la cellule hôte, l'ADN codant pour une ou plusieurs enzymes transportant le 2,5-DKG dans la cellule hôte.

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