Enhanced amplifiability of minute fixative-treated tissue...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2584723

The specification provides materials and methods for enhancing the amplifiability of nucleic acids that are present in minute amounts. Preferably, the materials and methods are utilized in combination with pathology techniques to amplify nucleic acids obtained from fixative and/or stained tissue or cell samples. The amplified nucleic acids can the be utilized for rendering a diagnosis or prognosis to the subject from which the tissue or cellular sample was obtained.

L'invention porte sur des matériaux et des procédés permettant de renforcer l'amplifiablité d'acides nucléiques présents en très petites quantités. Ces matériaux et procédés sont mis en oeuvre de préférence en liaison avec des techniques pathologiques de façon à amplifier des acides nucléiques obtenus à partir de fixatifs et/ou de tissus colorés ou d'échantillons de cellules. Ces acides nucléiques amplifiés permettent un diagnostic ou un pronostic du sujet sur lequel a été effectué le prélèvement.

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