Enhanced carotenoid accumulation in storage organs of...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 9/00 (2006.01)

Patent

CA 2203815

A transgenic higher plant, seed containing the transgene, and methods of providing enhanced carotenoid accumulation are disclosed. The transgenic higher plant has a genomic structural gene that encodes a chimeric polypeptide conjugate and over accumulates a colored native carotenoid in a preselected storage organ relative to the accumulation in a non-transgenic plant of the same type. Expression of the chimeric polypeptide is driven by a promoter operatively linked to that structural gene that provides storage organ-enhanced expression. The chimeric polypeptide has an N-terminal plastid transit peptide portion whose C-terminus is linked to the N-terminus of a non-higher plant phytoene synthase enzyme.

L'invention concerne une plante supérieure transgénique, une semence contenant le transgène, et des procédés permettant d'obtenir une accumulation accrue de caroténoïdes. La plante supérieure transgénique possède un gène de structure génomique qui code pour un conjugué de polypeptide chimère et accumule un excédent de caroténoïdes colorés à l'état natif dans un organe présélectionné de stockage par rapport à ce qu'une plante non transgénique du même type est en mesure d'accumuler. L'expression du polypeptide chimérique est commandée par un promoteur lié de manière active à ce gène de structure qui assure une expression améliorée par l'organe de stockage. Le polypeptide chimère possède une portion peptidique de transit de plaste N-terminal, dont l'extrémité C-terminale est liée à l'extrémité N-terminale d'une enzyme phytoène synthase d'une plante inférieure.

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