Enhanced cellular assay method for use in flow cytometry or...

G - Physics – 01 – N

Patent

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G01N 15/14 (2006.01) G01N 33/566 (2006.01)

Patent

CA 2526868

This invention relates to the field of biological assays where cells can be classified and enumerated using flow cytometry optical instrumentation. The invention combines information from multi-angle, light scatter from the cell itself and multi-angle light scatter from small, optically resonant particles that are selectively bound to surface molecules on the cell to carry out classification and enumeration. This light scatter method enables an instrumentation system that is simple to use, inexpensive to build, and mechanically robust; making it suitable for use in remote clinical environments.

L'invention concerne le domaine des dosages biologiques servant à classifier et à recenser des cellules au moyen d'instruments optiques de cytométrie de flux. La présente invention associe des informations de diffuseur de lumière à angles multiples provenant de la cellule elle-même et des informations de diffuseur de lumière à angles multiples provenant de petites particules à résonance optique qui sont fixées de manière sélective sur des molécules de surface de la cellule afin de réaliser la classification et le recensement. Cette méthode par diffuseur de lumière permet d'obtenir un système d'instruments simple à utiliser, peu onéreux à fabriquer et solide du point de vue mécanique. Ce système d'instruments peut être utilisé dans des environnements cliniques à distance.

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