C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 9/88 (2006.01) C12N 15/60 (2006.01) C12N 15/74 (2006.01) C12P 7/16 (2006.01)
Patent
CA 2736420
Lactic acid bacterial (LAB) cells were modified such that they have a specific activity of dihydroxy-acid dehydratase enzyme activity that is increased to about 0.1 µmol min-1 mg-1. LAB cells with even higher activities of 0.2 to 0.6 µmol min-1 mg-1 of DHAD activity were obtained. These modified cells may be used to produce isobutanol when additional isobutanol biosynthetic pathway enzymes are expressed.
Linvention concerne des cellules bactériennes de lacide lactique (LAB) modifiées de manière à présenter une activité spécifique de lenzyme dihydroxy-acide déshydratase qui soit augmentée denviron 0,1 µmol min-1 mg-1. Des cellules LAB présentant des activités DHAD encore plus élevées de 0,2 à 0,6 µmol min-1 mg-1 ont été obtenues. Ces cellules modifiées peuvent être utilisées pour produire de lisobutanol lorsque des enzymes de la voie biosynthétique de lisobutanol supplémentaires sont exprimées.
Paul Brian James
Suh Wonchul
Butamax(tm) Advanced Biofuels Llc
Torys Llp
LandOfFree
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