Enhanced expression of heterologous proteins in recombinant...

Details Enhanced expression of heterologous proteins in recombinant... Enhanced expression of heterologous proteins in recombinant...

C - Chemistry, Metallurgy

12

N

195/40, 195/1.29

C12N 1/38 (2006.01) C07K 14/02 (2006.01) C12N 1/16 (2006.01) C12N 15/67 (2006.01) C12N 15/81 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2022109

0014/FSC8 17893 TITLE OF THE INVENTION ENHANCED EXPRESSION OF HETEROLOGOUS PROTEINS IN RECOMBINANT HOSTS IN A NON-GROWTH MEDIA ABSTRACT OF THE DISCLOSURE The present invention relates to a fermentation process for producing recombinant proteins in a recombinant host strain. The process can be adapted for organisms containing a mutation in cell, wall and/or plasma membrane biosynthesis (e.g. mnn9) in a non-growth media. An example of such a strain is described in Merck's Application serial number 202,764, filed June 3, 1988. Additionally, the process can be adapted for organisms containing "wild-type" cell walls and plasma membrane.

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