Enhanced expression of human or humanized immunoglobulin in...

C - Chemistry – Metallurgy – 07 – K

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Details

C07K 16/00 (2006.01) A01K 67/027 (2006.01) C07K 14/705 (2006.01) C12N 15/62 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2661848

The present invention describes transgenic animals with human(ized) immunoglobulin loci and transgenes encoding human(ized) Ig.alpha. and/or Ig.beta. sequences. Of particular interest are animals with transgenic heavy and light chain immunoglobulin loci capable of producing a diversified human(ized) antibody repertoire that have their endogenous production of Ig and/or endogenous Ig.alpha. and/or Ig.beta. sequences suppressed. Simultaneous expression of human(ized) immunoglobulin and human(ized) Ig.alpha. and/or Ig.beta. results in normal B-cell development, affinity maturation and efficient expression of human(ized) antibodies.

La présente invention concerne des animaux transgéniques possédant des loci d'immunoglobulines humaines (humanisées) et des transgènes codant des séquences d'Ig.alpha. et/ou d'Ig.beta. humaines (humanisées). L'invention concerne en particulier des animaux possédant des loci d'immunoglobulines à chaînes lourdes et légères transgéniques capables de produire un répertoire d'anticorps humains (humanisés) diversifié dont la production endogène d'Ig et/ou de séquences d'Ig.alpha. et/ou d'Ig.beta. endogènes est supprimée. L'expression simultanée d'immunoglobuline humaine (humanisée) et d'Ig.alpha. et/ou d'Ig.beta. humaines (humanisées) permet d'obtenir un développement normal de lymphocytes B, une maturation d'affinité et une expression efficace d'anticorps humains (humanisés).

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