Enhanced in vitro synthesis of active proteins containing...

Details Enhanced in vitro synthesis of active proteins containing... Enhanced in vitro synthesis of active proteins containing...

C - Chemistry, Metallurgy

12

P

C12P 21/06 (2006.01) A61K 38/00 (2006.01) C07K 1/00 (2006.01) C07K 1/113 (2006.01)

Patent

CA 2419996

Compositions and methods are provided for the enhanced in vitro synthesis of polypeptides containing disulfide bonds. In order to improve the performance of in vitro protein synthesis reactions, pre-treatment and redox buffering of the reaction mix is performed in order to optimize the redox potential. Exogenous enzymes that enhance protein folding and disulfide bond formation may also be added to the reaction.

Cette invention concerne des compositions et des méthodes propres à améliorer la synthèse in vitro de polypeptides renfermant des liaisons disulfure. Pour améliorer les réactions de synthèse protéique in vitro, on procède à un prétraitement et à un tamponnage d'oxydo-réduction du mélange de réaction afin d'optimiser le potentiel d'oxydo-réduction. Il es également possible d'ajouter à la réaction des enzymes exogènes qui favorisent le repliement des protéines et la formation de liaisons disufure.

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