C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
167/43, 150/3.3
C12Q 1/28 (2006.01) G01N 33/58 (2006.01)
Patent
CA 1200486
ABSTRACT An enhanced luminescent or luminometric assay, especially immunoassay, having a luminescent reaction between a peroxidase enzyme, an oxidant, a 6-hydroxybenzothiazole and a chemiluminescent 2,3-dihydro-1,4-phthalazinedione. The preferred materials are horse radish peroxidase as the enzyme, hydrogen peroxide as the oxidant, 6-hydroxybenzothiazole, 2-cyano-6-hydroxybenzothiazole or firefly luciferin as the benzothiazole and either luminol or isoluminol as the phthalazinedione. In a particularly preferred embodiment the horse radish peroxidase is coupled to an antibody to the substance to be assayed and thereby acts as the assay label in an immunoassay.
422693
Carter Timothy J.n.
Groucutt Carol J.
Stott Richard A.w.
Thorpe Gary H.g.h.
Whitehead Thomas P.
Fetherstonhaugh & Co.
National Research Development Corporation
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