Enhanced luminescent or luminometric assay

Details Enhanced luminescent or luminometric assay Enhanced luminescent or luminometric assay

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/28 (2006.01) G01N 33/533 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1217121

ABSTRACT An enhanced luminescent or luminometric assay, especi- ally immunoassay, having a luminescent reaction between a peroxi- dase enzyme, an oxidant, a chemiluminescent 2,3-dihydro-1,4- phthalazinedione and a sensitivity enhancer of general formula I, Image I The preferred materials are horse radish peroxidase as the enzyme, hydrogen peroxide as the oxidant, one of 4-iodophenol, 4-phenyl- phenol or 2-chloro-4-phenylphenol as the enhancer and either luminol or isoluminol as the phthalazinedione. In a particularly preferred embodiment the horse radish peroxidase is coupled to an antibody to the substance to be assayed and thereby acts as the assay label in an immunoassay.

446973

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