C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 3/00 (2006.01) A01N 1/02 (2006.01) B01D 71/70 (2006.01) C12M 1/00 (2006.01) C12M 1/04 (2006.01) C12M 3/02 (2006.01) C12N 1/00 (2006.01) C12N 5/00 (2006.01) C12Q 1/02 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2657232
Provided is a cell culture apparatus for culturing cells, that provides enhanced oxygen delivery and supply to cells without the need for stirring or sparging, Oxygen diffusions occurs on both sides of the culture vessel, top and bottom, A gas-permeable membrane that includes perfluorocarbons in its composition allows for the rapid, enhanced and uniform transfer of oxygen between the environment of cells or tissues contained in the cell culture container apparatus and the atmosphere of the incubator in which, the cell culture apparatus is incubated.
L'invention concerne un appareil de culture cellulaire pour la mise en culture de cellules, assurant une distribution en oxygène améliorée et une meilleure alimentation des cellules sans agitation ni barbotage. La diffusion d'oxygène se produit des deux côtés de la cuve de culture, en haut et en bas. Une membrane perméable au gaz comprenant des hydrocarbures perfluorés dans sa composition permet le transfert rapide, amélioré et uniforme d'oxygène entre l'environnement de cellules ou de tissus contenus dans l'appareil à contenant de culture cellulaire et l'atmosphère de l'incubateur dans lequel l'appareil de culture cellulaire est incubé.
Dominguez-Bendala Juan
Fraker Christopher A.
Inverardi Luca
Ricordi Camillo
Robic
University Of Miami
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