Enhanced phospholipid reduction and calcification mitigation...

A - Human Necessities – 61 – L

Patent

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Details

A61L 31/14 (2006.01) A61L 27/36 (2006.01) A61L 27/50 (2006.01) A61L 31/00 (2006.01)

Patent

CA 2490060

There is a need in the tissue treatment arts of methods for effectively mitigating calcification, reducing calcification and reducing phospholipid content of implanted bioprosthetic tissues. The invention provides an effective protocol for preparing biological tissue for incorporation as a bioprosthetic device. Disclosed herein is the discovery that calcification of biological tissue is mitigated and phospholipid content is reduced by including a step in the pre-implantation protocol whereby the biological tissue is treated with a surfactant and cross linking agent in the absence of a denaturant. Furthermore, the biological tissues prepared under this protocol are well suited for use in bioprosthetic devices.

Selon la présente invention, il est nécessaire de concevoir des procédés de traitement tissulaire permettant d'atténuer et de réduire efficacement la calcification de tissus bioprothétiques implantés ainsi que de réduire efficacement leur teneur en phospholipides. Cette invention concerne un protocole efficace de préparation de tissus biologiques destinés à être implantés en tant que dispositifs bioprothétiques. L'invention repose sur la découverte selon laquelle il est possible d'atténuer la calcification d'un tissu biologique et de réduire sa teneur en phospholipides, par insertion, dans le protocole pré-implantatoire, d'une étape consistant à traiter le tissu biologique au moyen d'un tensioactif et d'un agent de réticulation en l'absence d'un dénaturant. En outre, les tissus biologiques préparés dans le cadre dudit protocole sont particulièrement appropriés pour être utilisés en tant que dispositifs bioprothétiques.

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