C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
195/1.14, 195/1.
C12P 21/00 (2006.01) C07K 16/00 (2006.01) C12N 1/00 (2006.01) C12N 5/02 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01)
Patent
CA 1268436
ENHANCED PRODUCTION OF PROTEINACEOUS MATERIALS IN EUCARYOTIC CELLS ABSTRACT OF THE DISCLOSURE Disclosed is a method of enhancing expression of recombinant DNA in eucaryotic cells. A tissue specific enhancer element obtained from the genome of an organism and active in a selected tissue type is combined with a transcriptionally com- petent transcription unit comprising a promoter and exons encoding for the proteinaceous material of interest (or its precursor). This recombinant DNA is transfected into cells derived from the same tissue as the tissue in which the enhancer element normally functions to enhance expression of endogeneous DNA. The resulting transformants express the exons of the transcription unit at high levels as the enhancer element increases the copy number of mRNA. The enhancer element operates to increase transcription independent of its orientation and position provided it is located within an active region on the DNA, generally between about 1-10 kilobases (kb) from the 3' or 5' end of the transcription unit.
473753
Gillies Stephen D.
Tonegawa Susumu
Massachusetts Institute Of Technology
Riches Mckenzie & Herbert Llp
LandOfFree
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