Enhanced protein production in bacillus

C - Chemistry – Metallurgy – 12 – N

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C12N 15/09 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2709804

The present invention relates to cells that have been genetically manipulated to have an altered capacity to express and/or produce proteins of interest. In particular, the present invention relates to modified host cells of Gram-positive microorganisms, such as Bacillus species that are capable of overexpressing ymaH. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode YmaH, and the modified host cells comprising them. In particular, the present invention relates to compositions and methods of overexpressing YmaH for enhancing the expression and production of proteins of interest (e.g., proteases) in Bacillus species.

La présente invention concerne des cellules qui ont été génétiquement manipulées pour modifier leur capacité à exprimer et/ou à produire des protéines d'intérêt. En particulier, la présente invention concerne des cellules hôtes modifiées de microorganismes à Gram positif, comme des espèces Bacillus qui peuvent surexprimer YmaH. L'invention comprend des produits de synthèse de polynucléotides et des vecteurs d'expression qui contiennent des séquences de polynucléotides qui codent YmaH, ainsi que les cellules hôtes modifiées qui les comprennent. En particulier, la présente invention concerne des compositions et des procédés de surexprimer YmaH pour améliorer l'expression et la production de protéines d'intérêt (par ex., des protéases) dans des espèces Bacillus.

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