Enhanced stimulation of erythropoiesis

A - Human Necessities – 61 – K

Patent

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Details

A61K 38/42 (2006.01) A61K 31/555 (2006.01) A61K 38/18 (2006.01) A61K 47/48 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2263978

The present invention is directed to compositions comprising heme-containing components and to methods that stimulate erythropoiesis comprising administration of a composition of the invention, such as purified hemoglobin. Surprisingly, heme-containing components such as hemoglobin can induce erythopoiesis in the presence of decreased concentrations of erythropoietin (Epo) and, in fact, function synergistically with Epo. Further, heme- containing components can be used alone or in conjunction with Epo for the treatment of anemias and other disorders due to decreases in erythropoietin or iron. It has been discovered that, in the presence of Epo, both hemoglobin and cross-linked hemoglobin can compensate for the reduction in erythroid cell growth and differentiation that occurs in the presence of reduced concentrations of Epo. The effect is specific to erythropoiesis, as evidenced by a lack of growth of non-erythroid progenitors such as CFU-GM.

La présente invention porte sur des compositions ayant des constituants contenant des hématies, et sur des procédés de stimulation de l'érythropoièse consistant à administrer une composition de l'invention, telle que de l'hémoglobine purifiée. Curieusement, des constituants contenant des hématies, tels que l'hémoglobine, peuvent favoriser l'érythropoièse en présence de concentrations réduites d'érythropoïétine et, en fait, fonctionnent de manière synergique avec l'érythropoïétine. En outre, des constituants contenant des hématies peuvent être utilisés seuls ou en combinaison avec des érythropoïétines pour traiter des anémies ou d'autres troubles associés à des baisses d'érythropoïétine ou de fer. On a découvert que, en présence d'érythropoïétines, aussi bien l'hémoglobine que l'hémoglobine réticulée peuvent compenser la baisse de croissance des érythrocytes et la différenciation qui survient en présence de concentrations réduites d'érythropoïétines. L'effet est spécifique à l'érythropoièse, comme l'atteste l'absence de croissance de progéniteurs non érythrocytaires tels que CFU-GM.

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