C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/82 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C12N 5/14 (2006.01)
Patent
CA 2331375
A method for increasing the mass of a storage organ of a plant comprising transforming the plant with at least one heterologous gene that encodes an enzyme that results in NAD(P)H consumption is disclosed. Preferably the method comprises transforming the plant with a gene that encodes an enzyme that is directly involved in NAD(P)H consumption. Such plants are characterized as having an increase in the mass of the storage organs. The enzymes include but are not limited to alcohol dehydrogenase, glutathione reductase, dehydroascorbate reductase, monodehydroascorbate reductase, mitochondrial alternative oxidase, NADH oxidase and NADPH oxidase. Vector and transformed plants are also disclosed.
L'invention concerne un procédé d'augmentation de la masse d'un organe de stockage d'une plante consistant à transformer la plante avec au moins un gène hétérologue codant pour une enzyme entraînant une consommation de NAD(P)H. Ce procédé consiste de préférence à transformer la plante avec un gène codant pour une enzyme directement impliquée dans la consommation de NAD(P)H. De telles plantes sont caractérisées par une augmentation de la masse des organes de stockage. Les enzymes contiennent, entre autres, de l'alcooldéshydrogénase, de la glutathion réductase, de la déshydroascorbate réductase, de la monodéshydroascorbate réductase, une oxydase alternative mitochondriale, une NADH oxydase et une NADPH oxydase. Font aussi l'objet de cette invention des vecteurs et des plantes transformées.
Bowley Stephen R.
Jones Kim S.
Mckersie Bryan D.
Samis Karen
Gowling Lafleur Henderson Llp
University Of Guelph
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