Enhanced yeast transcription employing hybrid promoter...

Details Enhanced yeast transcription employing hybrid promoter... Enhanced yeast transcription employing hybrid promoter...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.2

C12N 15/81 (2006.01) C07K 14/02 (2006.01) C07K 14/81 (2006.01) C12N 9/02 (2006.01)

Patent

CA 1281671

ENHANCED YEAST TRANSCRIPTION EMPLOYING HYBRID PROMOTER REGION CONSTRUCTS ABSTRACT OF THE DISCLOSURE Yeast promoters of glycolytic enzymes are modified by isolating a fragment encompassing the RNA polymerase binding site and joining to the 5' end of this fragment a DNA sequence providing for enhanced inducible or constitutive transcription of a structural gene. Constructs are prepared for efficient expression of foreign genes in yeast. Yeast strains 2150-2-3(pCl/1GAPSOD) and AB110(pCl/1GAPATi9), producing human .alpha.1-antitrypsin and superoxide dismutase, were deposited at the A.T.C.C. on May 9, 1984 and given Accession Nos. 20708 and 20709, respectively; and 2150-2-3(GAP5), 2150-2-3(Pyk5) and 2150-2-3(PHO5GAP1), expressing Hepatitis B surface anti- gen, were deposited at the A.T.C.C. on May 9, 1984 and given Accession Nos. 20705, 20706 and 20707, respective- ly.

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