C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12Q 1/42 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2221509
A method of preparing a homogeneous alkaline phosphatase--oligonucleotide probe conjugate having high specific enzyme activity for use in nucleic acid hybridization assays is disclosed. Methods and compositions for enhancing the chemiluminescence from a stable 1,2-dioxetane triggered to produce a chemiluminescence are also disclosed. Indirect, competitive nucleic acid hybridization assay formats are also described that employ these methods and compositions.
L'invention porte sur un procédé de préparation d'un conjugué sonde homogène de phosphatase alcaline/oligonucléotide, présentant une activité enzymatique spécifique, et qui s'utilise dans des dosages d'hybridation d'acides nucléiques; sur un procédé et des compositions renforçant la chimioluminescence d'une 1, 2 dioxétane stable pouvant être déclenchée pour produire la chimioluminescence; et sur des types de dosages d'hybridation d'acides nucléiques indirects et concurrents recourant à ces procédés et compositions.
Anderson Mary L.
Gagne Julio C.
Ludtke Douglas N.
Sheridan Patrick J.
Borden Ladner Gervais Llp
Chiron Corporation
Siemens Healthcare Diagnostics Inc.
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