Enhancement of expression of a single-stranded, heterologous...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/864 (2006.01) C12N 5/10 (2006.01) C12N 7/01 (2006.01) C12N 15/86 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2379166

Recombinant viral vectors, especially parvovirus such as adeno-associated virus (AAV) vectors, capable of enhanced expression of heterologous sequences, and methods for their construction and use, are provided. The vectors have a structure, or are capable of rapidly adopting a structure, which involves intrastrand base pairing of at least one region in a heterologous sequence.

L'invention concerne des vecteurs viraux de recombinaison, en particulier, des parvovirus, tels que des vecteurs du virus associé aux adénovirus (AAV), permettant une expression accrue des séquences hétérologues, ainsi que des procédés de production et d'utilisation de ceux-ci. Ces vecteurs ont une structure, ou sont capables d'adopter rapidement une structure, qui comprend l'appariement des bases intrabrines d'au moins une zone dans une séquence hétérogène.

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