Enhancement of freezing tolerance in transgenic strawberry

A - Human Necessities – 01 – N

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A01N 65/44 (2009.01) A01H 5/00 (2006.01) A01P 15/00 (2006.01) A01P 21/00 (2006.01) A23B 7/00 (2006.01) C07K 14/415 (2006.01) C12N 15/00 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2321944

The present invention relates to engineering cells, tissues and organs, from animals or plants for low temperature, dehydration or salt tolerance by using a cold- regulated gene from cold-tolerant plants. The invention also relates to the method used to efficiently transform and generate stable transgenic strawberry plants. This method was efficient in producing a cold-tolerant strawberry using a gene from a cold tolerant species. The gene encodes a protein involved in protecting the cellular membrane against osmotic stress caused by freezing, salt, and dehydration. The gene product could be useful in protecting leaves flowers and fruit against these osmotic stresses. This will result in extending shelf life of these fresh produce. The invention also teach the use of cold-regulated genes or their products to protect different molecules, cells, tissues and organs during storage at low temperature.

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