Enhancement of gene expression

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/67 (2006.01) C12N 9/00 (2006.01) C12N 15/29 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2252366

A method for enhancing the expression of a selected gene in an organism while avoiding or reducing co-suppression involves the synthesis of a DNA which is altered in nucleotide sequence and is capable of expression of a protein, ideally identical to that of a protein already expressed by a DNA already present in the organism. This method ensures that sequence similarity between the two genes is reduced enough to eliminate the phenomenon of co-suppression, allowing the over-expression of a specific protein. The method is particularly suitable in plants.

Un procédé qui permet de renforcer l'expression génique dans un organisme en même temps qu'il évite ou réduit la suppression conjointe implique la synthèse d'un ADN qui est modifié au niveau d'une séquence nucléotidique et peut exprimer une protéine théoriquement identique à celle d'une protéine déjà exprimée par un ADN déjà présent dans l'organisme. Ce procédé permet d'assurer que la similarité de séquences entre les deux gènes est suffisamment limitée pour éliminer le phénomène de suppression conjointe et permettre la surexpression d'une protéine spécifique. Ce procédé convient particulièrement à une utilisation pour les végétaux.

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