Enhancement of nucleic acid hybridization by anionic polymers

Details Enhancement of nucleic acid hybridization by anionic polymers Enhancement of nucleic acid hybridization by anionic polymers

C - Chemistry, Metallurgy

12

N

195/1.12

C12N 15/00 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 1254526

ABSTRACT OF THE DISCLOSURE The rate of hybridization between two complementary polynucleotide segments in an aqueous medium is increased by the presence of the anionic polymers polyacrylate and polymethacrylate. The acceleration effect is particularly useful in nucleic acid hybridization assays involving immobilization of sample nucleic acids and the use of labeled probes. Nonspecific binding of probe to nitrocellulose supports is substantially lower in the presence of the present polymers than in the presence of the prior art accelerator dextran sulfate. Polyacrylate is particularly advantageous since it has been found to be effective at low concentrations and is significantly less expensive than the prior art compound.

495025

Affiliated with

Also associated with

Rating

Enhancement of nucleic acid hybridization by anionic polymers does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enhancement of nucleic acid hybridization by anionic polymers, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enhancement of nucleic acid hybridization by anionic polymers will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1222557