Enhancement of the immune response for vaccine and gene...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/79 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2365907

Expression cassettes are provided comprising a promoter operably linked to a nucleic acid molecule which, when transcribed in vivo, forms double-stranded RNA that induces the production of interferon. Expression cassettes also are provided comprising a promoter operably linked to a ribozyme or antisense nucleic acid molecule which, when transcribed in vivo, forms a ribozyme or antisense RNA molecule that stimulates an immune response. In addition, expression cassettes are provided comprising a promoter operably linked to a ribozyme or antisense nucleic acid molecule which, when transcribed in vivo, stimulates apoptosis. Finally, gene delivery vectors are provided which contain such expression cassettes, host cells containing the gene delivery vectors, and methods of utilizing the expression cassettes, gene delivery vectors, and host cells.

L'invention concerne des cassettes d'expression contenant un promoteur lié fonctionnellement à une molécule d'acide nucléique qui, après transcription in vivo, forme un ARN bicaténaire induisant la production d'interférons. L'invention concerne également des cassettes d'expression contenant un promoteur lié fonctionnellement à une molécule de ribozymes ou d'acide nucléique anti-sens qui, après transcription in vivo, forme une molécule de ribozymes ou d'ARN anti-sens stimulant une réponse immune. De plus, l'invention concerne des cassettes d'expression contenant un promoteur lié fonctionnellement à une molécule de ribozymes ou d'acide nucléique anti-sens qui, après transcription in vivo, stimule l'apoptose. Enfin, l'invention a trait à des vecteurs d'apport de gènes contenant ces cassettes d'expression, des cellules hôtes contenant les vecteurs d'apport de gènes, et des méthodes d'utilisation des cassettes d'expression, des vecteurs d'apport de gènes et des cellules hôtes.

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