Enhancer elements for increased translation in plant plastids

Details Enhancer elements for increased translation in plant plastids Enhancer elements for increased translation in plant plastids

C - Chemistry, Metallurgy

12

N

C12N 15/15 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 14/61 (2006.01) C07K 14/81 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/18 (2006.01) C12N 15/54 (2006.01) C12N 15/62 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2333308

Provided are methods for increasing the production of protein in a plant cell by transforming plastids of plant cells with a construct comprising a promoter functional in a plant plastid, a ribosome binding site, DNA sequence of interest and a transcription termination region, and growing plant cells comprising the transformed plastids under conditions wherein the DNA encoding sequence is transcribed in the plastid. Also provided are methods for increasing protein production by fusing a coding sequence to a gene of interest to a secondary protein for cleavage or targetting of the protein of interest within the plastid, whereby high levels of expression of protein is achieved.

L'invention concerne des procédés permettant d'accroître la production de protéines dans une cellule végétale, comprenant la transformation des plastes des cellules végétales avec un gène recombiné comprenant un promoteur fonctionnel dans un plaste végétal, un site d'attachement des ribosomes, la séquence d'ADN choisie et une région de terminaison de transcription, suivie d'une culture des cellules végétales contenant les plastes transformés dans des conditions propres à assurer la transcription de la séquence de codage d'ADN dans le plaste. L'invention concerne en outre des procédés permettant d'accroître la production de protéines par la fusion d'une séquence codante d'un gène choisi avec une protéine secondaire en vue d'un clivage ou d'un ciblage de la protéine choisie à l'intérieur du plaste, ce qui entraîne de hauts niveaux d'expression de protéines.

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