Enhancing expression of value-added genes by transgenic...

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A01H 1/00 (2006.01) C12N 15/82 (2006.01) C12N 15/87 (2006.01)

Patent

CA 2640473

A method of enhancing expression of at least one desired gene product in a plant, comprises transgenically introducing a Tombusvirus pl9/R43W mutant gene (SEQ. ID NO: 3) into the plant, expressing at least one desired gene product in the plant, wherein expression of said product is susceptible to RNA silencing; and co-expressing the transgenically introduced pl9/R43W, to produce an amount of P19/R43W mutant protein (SEQ. ID NO: 4) sufficient to suppress silencing of expression of said desired gene product.

La présente invention concerne un procédé stimulant l'expression d'au moins un produit génique désiré dans une plante, qui consiste à : introduire de manière transgénique un gène mutant du Tombusvirus pl9/R43W (SEQ. ID N° 3) dans la plante ; exprimer au moins un produit génique souhaité dans la plante, l'expression dudit produit étant susceptible de taire l'ARN ; et co-exprimer le pl9/R43W introduit de manière transgénique en vue de produire une quantité suffisante de protéines mutantes du P19/R43W (SEQ. ID N° 4) pour supprimer le silençage de l'expression dudit produit génique souhaité.

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