Enhancing the circulating half-life of antibody-based fusion...

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 19/00 (2006.01) A61K 47/48 (2006.01) C07K 5/103 (2006.01) C07K 14/52 (2006.01) C07K 14/525 (2006.01) C07K 14/55 (2006.01) C07K 16/00 (2006.01) C07K 16/28 (2006.01) C07K 16/30 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2399832

Disclosed are compositions and methods for enhancing the circulating half-life of antibody-based fusion proteins. Disclosed methods and compositions rely on altering the amino acid sequence of the junction region between the antibody moiety and the fused protein moiety in an antibody-based fusion protein. An antibody-based fusion protein with an altered amino acid sequence in the junction region has a greater circulating half-life when administered to a mammal. Disclosed methods and compositions are particularly useful for reducing tumor size and metastasis in a mammal.

L'invention concerne des compositions et des méthodes permettant d'améliorer la demi-vie circulante de protéines de fusion à base d'anticorps. Ces méthodes et ces compositions consistent à modifier la séquence d'acide aminé de la région de jonction entre la fraction d'anticorps et la fraction de protéine fusionnée dans une protéine de fusion à base d'anticorps. Une protéine de fusion à base d'anticorps comportant une séquence d'acide aminé modifiée dans sa région de jonction possède une demi-vie circulante plus longue lorsqu'elle administrée à un mammifère. Ces méthodes et ces compositions sont notamment utiles pour réduire la taille des tumeurs et les métastases chez un mammifère.

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