Enone reductase

Details Enone reductase Enone reductase

C - Chemistry, Metallurgy

12

N

C12N 9/02 (2006.01) C12P 7/26 (2006.01)

Patent

CA 2474780

An isolated enone reductase characterized by a molecular mass of 61300 +/- 5000 Da; NADPH and NADH as co-factor; a temperature optimum of 55-60 ~C at pH 7.4 ; a pH optimum of 4.5-8.5 and substrate specificity on .prop.,.beta.- unsatured ketones; a process for producing it from a microorganism and a process for producing levodione from ketoisophorone using such reductase.

Réductase d'enone isolée caractérisée par une masse moléculaire de 61300 +/- 5000 Da; NADPH et NADH comme co-facteurs, un optimum de température de 55-60 DEG C à pH 7,4, un optimum de pH de 4,5-8,5 et une spécificité des substrats vis-à-vis des cétones alpha , beta - insaturés, processus pour la produire à partir d'un micro-organisme et processus pour produire du lévodione à partir de céto-isophorone en utilisant cette réductase.

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