C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/02 (2006.01) C12N 5/06 (2006.01)
Patent
CA 2668826
The present invention provides a novel method to isolate and expand pure progenitor/stem cells from a primary tissue explant, which produces a population enriched in multipotent functional progenitor/stem cells free of contaminating fibroblasts and other cell types. Cardiac progenitor/stem cells isolated by this method maintain their self-renewal and clonogenic character in vitro and differentiate into normal cells in myocardium, including cardiomyocytes, endothelial cells, and smooth muscle cells, after transplantation into ischemic hearts. The present invention also includes substantially pure populations of multipotent progenitor/stem cells, e.g., cardiac progenitor/stem cells, and their use to treat and prevent diseases and injuries, including those resulting from myocardial infarction.
La présente invention concerne un nouveau procédé permettant d'isoler et de développer ces cellules progénitrices ou souches pures à partir d'un explant tissulaire primaire, produisant ainsi une population de cellules progénitrices ou souches multipotentes exemptes de fibroblastes ou autres types de cellules contaminantes. Les cellules progénitrices ou souches cardiaques isolés selon ce procédé conservent in vitro leur caractère de renouvellement spontané et clonogène et se différencient dans le myocarde en cellules normales, dont notamment les cardiomocytes, les cellules endothéliales et les cellules de muscles lisses, après transplantation dans les cAEurs ischémiques. L'invention concerne également des populations essentiellement pures de cellules progénitrices ou souches multipotentes, par exemple les cellules progénitrices ou souches cardiaques, et leur utilisation pour traiter et prévenir des maladies et lésions, y-compris celles résultant de l'infarctus du myocarde.
Phillips Michael Ian
Tang Yao Liang
Gowling Lafleur Henderson Llp
Keck Graduate Institute
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