Enrichment of enzymatic cleavage products

Details Enrichment of enzymatic cleavage products Enrichment of enzymatic cleavage products

G - Physics

01

N

G01N 33/68 (2006.01) C07K 1/22 (2006.01) C12N 9/64 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2524680

The invention relates to a method for the enrichment, isolation and/or identification of cleavage products of at least one enzyme from a sample. According to the invention, an enzymatically inactive mutant of a protease is used as an affinity material, said mutant furthermore maintaining its specific substrate nature. At least one cleavage product of the protease of which the mutant is used, and at least one cleavage product of the enzyme of which the cleavage products are to be analysed, comprise at least one structural similarity.

L'invention concerne un procédé permettant d'enrichir, d'isoler et/ou d'identifier des produits de clivage d'au moins une enzyme à partir d'un échantillon. A cet effet, un mutant enzymatiquement inactif d'une protéase est utilisé comme matière d'affinité, ledit mutant conservant sa spécificité de substrat. Selon l'invention, au moins un produit de clivage de la protéase dont le mutant est utilisé et au moins un produit de clivage de l'enzyme dont les produits de clivage doivent être analysés présentent au moins une similitude structurelle.

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