Enrichment of hematopoietic stem cells from blood or bone...

C - Chemistry – Metallurgy – 12 – M

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C12M 3/00 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2211380

Process for enrichment of hematopoietic stem cells in a stem cell preparation e.g. bone marrow, umbilical cord blood and peripheral blood stem cell preparation. Non-stem cells, in particular monocytes and myeloid cells, are depleted from the preparation by adherence to an opsonized material, e.g. a scrubbed nylon fiber coated with human IgG and blocked with human serum albumin. Platelets are depleted from the stem cell preparation before contacting the opsonized material, e.g. by counterflow centrifugal elutriation. Alternatively, adherence of platelets to stem cells is prevented by the addition of a chelating substance to the medium in which the enrichment process is performed.

Procédé d'enrichissement des cellules souches hématopoïétiques dans une préparation de cellules souches, p. ex. préparations de cellules souches de moelle osseuse, de sang de cordon ombilical et de sang périphérique. Les cellules autres que les cellules souches, en particulier les monocytes et les cellules myéloïdes, sont enlevées de la préparation par adhésion à une matière opsonisée, p. ex. fibre de nylon brossée enduite d'IgG humaine et bloquée par de l'albumine sérique humaine. Les plaquettes sont enlevées de la préparation de cellules souches avant le contact avec la matière opsonisée, p. ex. par élutriation par centrifugation à contre-courant. Autre possibilité, on peut empêcher l'adhésion des plaquettes aux cellules souches par l'addition d'une substance chélatante dans le milieu dans lequel le procédé d'enrichissement est réalisé.

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