Entrapment of nucleic acid sequencing template in sample...

Details Entrapment of nucleic acid sequencing template in sample... Entrapment of nucleic acid sequencing template in sample...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) B01D 57/02 (2006.01) C12N 15/10 (2006.01) G01N 1/28 (2006.01) G01N 27/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2189381

A capillary electrophoresis system and method of electrokinetically loading a capillary electrophoresis sample into a separation medium in a capillary tube in which an entangled polymer matrix is formed having the sample embedded therein. The matrix has a mesh size effective to retard movement of macromolecules such as DNA sequencing templates through the matrix when an electric field is applied across the matrix. The entangled polymer matrix is formed by a linear polymer having a molecular weight of at least 20K Daltons. Furthermore, the invention includes stable denaturants useful for the electrophoresis of nucleic acids.

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