C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C12N 15/10 (2006.01) C12Q 1/70 (2006.01) G01N 27/447 (2006.01)
Patent
CA 2153175
A capillary electrophoresis system and method of electrokinetically loading a capillary electrophoresis sample into a separation medium in a capillary tube in which an entangled polymer matrix is formed having the sample embedded therein. The matrix has a mesh size effective to retard movement of macromolecules such as DNA sequencing templates through the matrix when an electric field is applied across the matrix. The entangled polymer matrix is formed by a linear polymer having a molecular weight of at least 20KDalrons.
Système d'électrophorèse capillaire et procédé de chargement électrocinétique d'un échantillon d'électrophorèse capillaire dans un milieu de séparation d'un tube capillaire, qui comprend la constitution d'une matrice de polymère enchevêtré dans laquelle l'échantillon est intégré. La matrice possède des mailles d'une taille permettant de retarder le déplacement à travers la matrice de macromolécules telles que des matrices de séquençage d'ADN, lorsqu'un champ électrique est appliqué sur la matrice. La matrice de polymère enchevêtré est formée d'un polymère linéaire ayant une masse moléculaire de 20 kilodaltons au moins.
Applied Biosystems Llc
Perkin-Elmer Corporation
Sim & Mcburney
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