Entrapped binding proteins as biosensors

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 17/00 (2006.01) C07K 17/04 (2006.01) C12P 39/00 (2006.01) C12Q 1/00 (2006.01) C12Q 1/54 (2006.01) G01N 27/00 (2006.01) G01N 31/00 (2006.01) G01N 31/22 (2006.01) G01N 33/66 (2006.01)

Patent

CA 2471889

The invention is directed to entrapped binding proteins, mutated binding proteins containing reporter groups, compositions of mutated binding proteins containing reporter groups in analyte permeable matrices, and their use as analyte biosensors both in-vitro and in vivo. The binding proteins are covalently bound to a fluorescein dye so that the concentration of an analyte (e.g., glucose) in a given sample is proportional to the fluorescence signal on a scale.

La présente invention concerne des protéines de liaison piégées, des protéines de liaison mutées contenant des groupes rapporteurs, des compositions de protéines de liaison mutées contenant des groupes rapporteurs dans des matrices d'analytes perméables, et leur utilisation en tant que biocapteurs d'analytes tant in vitro que in vivo. Les proteines de liaison sont liées par covalence à un colorant fluorescéinique de sorte que la concentration d'un analyte (par exemple, le glucose) dans un échantillon donné soit proportionnelle au signal de fluorescence sur une échelle.

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