Environmental biosensor for measuring air contaminants

Details Environmental biosensor for measuring air contaminants Environmental biosensor for measuring air contaminants

G - Physics

01

N

G01N 33/50 (2006.01) G01N 21/84 (2006.01)

Patent

CA 2321899

The invention concerns a biosensor comprising: a source (15) of cell culture for immune defence of the human organism, such as alveolar monocytes and macrophages, sensitive to said air contaminants; a probe for drawing (31, 32) working samples representing the contaminated atmosphere, and for delivering the samples in the form of a gas flow with controlled flow rate; an exposure cell (1) defining a sealed chamber (7) to be swept by a gas stream and whereof the base (2), made of a translucent material, receives in the form of a liquid sheet (9) in contact with said gas stream, said culture; means for rinsing (42, 45) said exposure cell base (2); and a system (3, 4, 5, 46, 47, 48, 39) for examining and displaying the cell base to produce images revealing the behavioural modifications of the monocytes and macrophages, for examples those concerning their mobility towards an attracting chemical agent deposited on said base (2) for this purpose. The invention is useful for fast and dependable characterisation of the harmfulness of air contaminants on human health.

Ce biocapteur comprend: une source (15) de culture de cellules de défense immunitaire de l'organisme humain, telles que des monocytes et macrophages alvéolaires, sensibles à ces aérocontaminants; une sonde de prélèvement (30, 12) d'échantillons d'analyse représentatifs de l'atmosphère contaminée, et apte à délivrer les échantillons sous forme d'un flux gazeux à débit contrôlé; une cellule d'exposition (1) définissant une enceinte étanche (7) destinée à être balayée par un courant gazeux et dont le fond (2), en matériau translucide, reçoit, sous forme d'une nappe liquide (9) au contact dudit courant gazeux, de ladite culture; des moyens de rinçage (42, 45) du fond (2) de ladite cellule d'exposition; et un système (3, 4, 5, 46, 47, 48, 39) d'inspection et de visualisation du fond de la cellule pour élaborer des images aptes à révéler des modifications comportementales des monocytes et macrophages, par exemple celles de leur mobilité en direction d'un agent chimio-attractif déposé à cet effet sur le fond (2). Application à la caractérisation rapide et fiable de la nocivité des aérocontaminants à l'égard de la santé humaine.

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