Enzymatic amidation of peptides

Details Enzymatic amidation of peptides Enzymatic amidation of peptides

C - Chemistry, Metallurgy

12

P

C12P 21/06 (2006.01) C07K 14/605 (2006.01) C12N 9/50 (2006.01)

Patent

CA 2349527

The invention provides a method of producing a polypeptide having a C-terminal .alpha.-carboxamide group. It particularly concerns an enzymatic modification of selected substrate polypeptides which result in cleavage of the substrate polypeptide to form a product peptide with a C-terminal arginine residue having an .alpha.-carboxamide group (C-terminal "Arg-NH2"). The method includes contacting an aqueous-based solution including (i) ammonia reagent and (ii) the substrate polypeptide with (iii) clostripain.

L'invention concerne un procédé permettant de produire un polypeptide présentant un groupe .alpha.-carboxamide C-terminal. Elle concerne en particulier une modification enzymatique de polypeptides substrats choisis qui produit un clivage du polypeptide substrat pour former un produit peptidique avec un résidu C-terminal arginine présentant un groupe .alpha.-carboxamide (C-terminal "Arg-NH¿2?"). Le procédé consiste en outre à mettre en contact une solution à base aqueuse comprenant (i) un réactif d'ammoniac et (ii) le polypeptide substrat avec (iii) du clostripain.

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