Enzymatic analysis using substrates that yield fluorescent...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/34 (2006.01) C07D 235/18 (2006.01) C07D 239/91 (2006.01) C07D 263/57 (2006.01) C07D 277/66 (2006.01) C07F 9/547 (2006.01) C07F 9/6512 (2006.01) C07F 9/6541 (2006.01) C07H 15/26 (2006.01) C07K 14/00 (2006.01) C07K 16/00 (2006.01) C12Q 1/42 (2006.01) C12Q 1/68 (2006.01) G01N 1/30 (2006.01) G01N 33/52 (2006.01) G01N 33/53 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2115461

Novel fluorescent precipitating substances made from a class of fluorophores, generally including quinazolinones (quinazo- lones), benzimidazoles, benzothiazoles, benzoxazoles, quinolines, indolines, and phenanthridines, having general formula (I), where carbon atoms of -C1=C2- are further joined so as to complete a first 5- or 6-membered aromatic ring which may con- tain at least one of the hetero atoms N, O or S, where carbon atoms of -C4-N=C3- are further joined so as to complete a second 5- or 6-membered aromatic ring that contains at least the nitrogen between C3 and C4 and may contain at least one additional hereto atom N, O or S, where the first and second aromatic rings may be joined by a 5- or 6-membered bridging ring that contains at least the C from the first aromatic ring and the C3 from the second aromatic ring, which bridging ring may be saturated or unsaturated and may contain a hetero atom N, O, or S, where each of the first and second aromatic rings may be fused to at least one additional aromatic ring that may contain at least one of the hetero atoms N, O or S, and where each of said aromatic rings may be further modified by substitution of any hydrogens on an aromatic carbon by sub- stituents that are halogen, nitro, cyano, aryl, lower alkyl (1-4 carbons), perfluoroalkyl (1-4 carbons), or alkoxy (1-4 carbons), or any combination thereof; and Xfl is covalently linked to the oxygen -O- at C1.

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