Enzymatic assay for carbon dioxide

Details Enzymatic assay for carbon dioxide Enzymatic assay for carbon dioxide

C - Chemistry, Metallurgy

12

Q

150/15.1

C12Q 1/32 (2006.01) C12Q 1/48 (2006.01) G01N 33/52 (2006.01) G01N 33/84 (2006.01)

Patent

CA 2036057

-0- ENZYMATIC ASSAY FOR CARBON DIOXIDE Abstract of the Disclosure A method for the assay of carbon dioxide in body fluids is disclosed. The method involves: a) mixing, at a pH in the range of 6.0 to 8.5, a measured amount of said body fluid with excess amounts of phosphoenolpyruvate; malate dehydrogenase; from 0.2 to 1.5 mmol/L of reduced nicotinamide adenine dinucleotide; from 0.5 to 300 U/L phosphoenolpyruvate carboxykinase; from 0.1 to 10.0 mmol/L of each of the cofactors magnesium and manganese ions; and from 0.1 to 10 mmol/L of a sulfhydryl agent and b) spectrophotometrically measuring the change in the concentration of the reduced form of nicotinamide adenine dinucleotide in the mixture at a substantially constant temperature and pH in the range of 6.0 to 8.5.

Affiliated with

Also associated with

Rating

Enzymatic assay for carbon dioxide does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Enzymatic assay for carbon dioxide, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Enzymatic assay for carbon dioxide will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1363113