Enzymatic assays for screening anti-cancer agents

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/37 (2006.01) A61P 35/00 (2006.01) C07K 14/47 (2006.01) C12Q 1/25 (2006.01) C12Q 1/42 (2006.01) G01N 33/574 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2408002

Methods of identifying anti-cancer agents are provided based on ligand binding or enzymatic activity of TIP49 family members. The assays may include the measurement of ATPase and/or helicase activity. Also provided are anti-cancer agents identified by the screening methods of the invention, as well as the use of the agent for the prophylaxis or treatment of cancer. A complex of a TIP49 family member with various proteins that regulate transcription is also provided.

L'invention concerne des méthodes d'identification d'agents anticancéreux basées sur une activité de liaison par ligand ou une activité enzymatique de membres de la famille TIP49. Les tests peuvent comporter la mesure de l'activité ATPase et/ou hélicase. L'invention concerne aussi des agents anticancéreux identifiés à l'aide des méthodes de criblage de l'invention, ainsi que l'utilisation de ces agents en prophylaxie ou en traitement de cancer. Elle concerne enfin un complexe d'un membre de la famille TIP49 avec différentes protéines qui régulent la transcription.

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