Enzymatic cofactor cycling using soluble pyridine nucleotide...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 1/21 (2006.01) C12N 9/02 (2006.01) C12P 17/18 (2006.01) C12Q 1/26 (2006.01)

Patent

CA 2269640

In an enzymic reaction involving a pyridine nucleotide cofactor, an enzyme is used that has sequence of greater than 70 % identity to SEQ ID No: 2 and capable of transferring reducing equivalents between pyridine nucleotide cofactors. Alternatively, a cell transformed to express the enzyme may be used.

Dans une réaction enzymatique mettant en jeu un cofacteur pyridino-nucléotidique, on utilise une enzyme qui possède une séquence ayant une identité avec SEQ ID n DEG 2 supérieure à 70 % et qui est capable de transférer des équivalents réducteurs entre les cofacteurs pyridino-nucléotidiques. Il est également possible d'utiliser une cellule transformée pour exprimer l'enzyme.

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