Enzymatic composition for ethanol assay

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/32 (2006.01) C12N 9/96 (2006.01)

Patent

CA 2057395

RAN 4091/18 Abstract An enzymatic assay composition for ethanol determination is provided comprising (a) enzyme alcohol dehydrogenase; (b) coenzyme nicotinamide adenine dinucleotide; and (c) a compound of the formula H2N-(CH2)n-CHR-CH2-NH2 I where R is H, CH3, or OH; and n is 0, 1, 2, 3 or 4 with the proviso that when R is OH, n cannot be 0. Compound I acts as a buffer and a trapping agent for a product of the enzymatic reaction. Long stability of the assay composition is achieved.

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