Enzymatic determination of theophylline

Details Enzymatic determination of theophylline Enzymatic determination of theophylline

C - Chemistry, Metallurgy

12

Q

150/15, 324/23,

C12Q 1/00 (2006.01) C12Q 1/26 (2006.01) C12Q 1/32 (2006.01) C12Q 1/62 (2006.01)

Patent

CA 1339794

The present invention provides a new methodology and test composition for determining the presence of theophylline in test samples. The methodology employs enzymes that utilize or recognize theophylline as a substrate to measure the concentration thereof in samples, including body fluids. This new approach utilizes enzymes as opposed to traditional methods which use antibodies for the recognition of theophylline. The enzymatic approach to theophylline determination is quick, simple and convenient and allows test systems to be made in liquid as well as in dry-chemistry formats. Various protocols, systems or methodologies may be used for assaying and relating the results to the amount of theophylline present. Methods for obtaining theophylline utilizing or recognizing enzymes are also described.

590650

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