Enzymatic ethanol test

Details Enzymatic ethanol test Enzymatic ethanol test

C - Chemistry, Metallurgy

12

Q

150/15.1, 150/3.

C12Q 1/26 (2006.01) C12Q 1/00 (2006.01) C12Q 1/28 (2006.01)

Patent

CA 1254117

ABSTRACT OF THE DISCLOSURE The present invention provides a stable, conven- ient solid state test device for the determination of ethanol in an aqueous test sample, a method for its pre- paration and a method for its use. The use of carrier matrix incorporated with alcohol oxidase, a peroxida- tively active substance and a suitable chromogenic indicator for the determination of ethanol in aqueous samples such as body fluid samples (e.g., serum, urine or saliva) is part of the invention. The invention provides a method of incorporating the enzyme alcohol oxidase and a peroxidatively active substance into a carrier matrix with a chromogenic indicator system capable of providing a detectable response to the presence of at least 100 mg/dL ethanol in less than about 5 minutes. The method involves either a) the use of a specialized incorporation procedure or b) the addition of an azide to the test device. Either method overcomes the "false positive" problem seen when solu- tion assay reagents are incorporated at the increased concentrations necessary to provide a test device sensitive to the at least 100 mg/dL ethanol.

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