C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 19/14 (2006.01) C08B 37/00 (2006.01) C12N 9/38 (2006.01)
Patent
CA 2066036
2066036 9104335 PCTABS00003 Selectively digesting the Group B Streptococcus type III (GBS-III) polysaccharide capsule (PC) to yield fragments consisting of intact GBS-III polysaccharide capsule repeating units. GBS-III polysaccharide capsule is digested by reacting it with a bacterial endo-.beta.-galactosidase that is specific for the galactose-glucose glycosidic linkage in the GBS-III CP backbone, and that substantially does not hydrolyse any other GBS-III CP glycosidic linkages. The resulting polysaccharide fragments are recovered and are immunologically useful. The endo-.beta.-galactosidase is produced in a multiphase culture that comprises: i) a liquid aqueous phase; ii) a second phase that is aqueous insoluble and in contact with the liquid aqueous phase at an interface therebetween, and iii) bacterial cells positioned generally in the liquid aqueous phase and at the interface between the liquid aqueous phase and the second phase. The endo-.beta.-galactosidase is recovered from the liquid aqueous phase.
Kasper Dennis L.
Paoletti Lawrence C.
Wessels Michael
Brigham And Women's Hospital
Smart & Biggar
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