Enzymatic hydrolysis of granular starch directly to glucose

Details Enzymatic hydrolysis of granular starch directly to glucose Enzymatic hydrolysis of granular starch directly to glucose

C - Chemistry, Metallurgy

12

N

195/124, 195/138

C12N 9/30 (2006.01) C12N 9/24 (2006.01) C12N 9/34 (2006.01) C12P 19/20 (2006.01)

Patent

CA 1282724

ENZYMATIC HYDROLYSIS OF GRANULAR STARCH DIRECTLY TO GLUCOSE ABSTRACT OF THE DISCLOSURE It is discovered that certain fungi, particu- larly members of the genus Humicola, secrete an enzyme mixture or preparation that hydrolyzes raw or granular starch directly to glucose without producing substantial amounts of limit dextrins or tri or higher number saccharides. The fungi is germinated and fermented on carbohydrate substrates, producing a broth exhibiting raw starch hydrolyzing activity. The broth is separated from the fungi and is added to a slurry of raw starch to hydrolyze the same to glucose. To produce glucose more efficiently, mutations have been induced in Humicola, and certain mutants produce elevated titers of raw starch hydrolyzing activity.

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