Enzymatic hydrolysis of lignocellulosic feedstocks using...

C - Chemistry – Metallurgy – 12 – P

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C12P 19/02 (2006.01) C12N 9/24 (2006.01) C12N 9/42 (2006.01) C12P 19/00 (2006.01)

Patent

CA 2697791

Provided is an enzyme mixture for hydrolyzing a pretreated lignocellulosic feedstock to soluble sugars. The enzyme mixture comprises EG4 at a fractional concentration (fEG4) of about 0.09 to about 0.91 (w/w), Swollenin at a fractional concentration (f Swo1) of about 0 to about 0.91(w/w), and Cip1 at a fractional concentration (f Cip1) of 0 to about 0.42 measured relative to all of the accessory enzymes present in the enzyme mixture. Also provided are processes for converting a pretreated lignocellulosic feedstock to soluble sugars using the enzyme mixtures, and methods of using and producing such enzyme mixtures.

La présente invention concerne un mélange d'enzymes permettant l'hydrolyse d'une charge lignocellulosique prétraitée en sucres soluble. Le mélange d'enzymes comporte EG4 à une concentration fractionnaire (fEG4) comprise entre environ 0.09 et environ 0,91 en poids, de la Swolennine à une concentration fractionnaire (fSwo1) comprise entre environ 0 et environ 0,91 en poids, et Cip1 à une concentration fractionnaire (fCip1) comprise entre environ 0 et 0,42 mesurée par rapport à la totalité d'enzymes accessoires dans le mélange d'enzymes. L'invention concerne également des procédés de conversion d'une charge lignocellulosique prétraitée en sucres solubles au moyen de mélanges d'enzymes, et des procédés d'utilisation et de production de tels mélanges d'enzymes.

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