Enzymatic immunoassay

Details Enzymatic immunoassay Enzymatic immunoassay

G - Physics

01

N

167/45

G01N 33/53 (2006.01) G01N 21/64 (2006.01) G01N 33/557 (2006.01)

Patent

CA 1280692

Abstract of the Disclosure: Enzymatic immunoassay for estimating the con- centration of antigen or antibody comprising bringing an antigen-antibody complex, which is labelled by an enzyme and is present heterogeneously in a solution, into con- tact with a substrate where pH of the solution is so adjusted as to be suitable for the enzyme to be activated and also for measuring the fluorescence of the substrate, measuring the time-variation of fluorescent intensity of the substrate produced by the enzyme reaction, and estimating the concentration of the antigen or the antibody from the slope of the substantially straight linear portion, as estimated from at least two points, on a characteristic curve representing variation of the fluorescence intensity.

517031

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