C - Chemistry – Metallurgy – 12 – P
Patent
C - Chemistry, Metallurgy
12
P
C12P 21/02 (2006.01) C07K 5/04 (2006.01) C07K 5/072 (2006.01) C07K 7/06 (2006.01) C07K 14/70 (2006.01) C12M 1/40 (2006.01) C12P 41/00 (2006.01)
Patent
CA 2054754
2054754 9012883 PCTABS00002 The present invention discloses a method for the enzymatic synthesis of a peptide. A protected peptide having a C-terminal carboxylate group or a protected N-acyl amino acid having an alpha carboxylate group is reacted with a protected peptide having an N-terminal ammonium group or a protected amino acid having an alpha ammonium group in the presence of a condensation enzyme under conditions in which the carboxylate group and the ammonium group condense to form a protected, uncharged, peptide product. This peptide product is transported across a water-immiscible hydrophobic phase disposed in a hollow fiber lumen into an aqueous product phase and is removed from the aqueous product phase to prevent back diffusion across the water-immiscible hydrophobic phase. Reverse osmosis and other separation techniques may be utilized to remove the peptide product from the product phase. The water-immiscible hydrophobic phase functions as an ion rejection membrane separating the aqueous reaction phase from the product phase creating oil/water interfaces with each of the aqueous phases. The present invention can be practised either in ILM's supported on microporous hollow fiber modules, or in oil/water contactors made of hydrophilic hollow fiber. An integrated process for the enzymatic resolution of D-, L-phenylalanine isopropyl ester and the racemization and recycling of D-phenylalanine isopropyl ester is also disclosed.
Brose Daniel J.
Iacobucci Guillermo A.
Ray Roderick J.
Van Eikeren Paul
Coca-Cola Company (the)
Sim & Mcburney
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